Enzyme Technologies for Pharmaceutical and Biotechnological Applications 

0-8247-0549-1

978-0-8247-0549-7

H.A. Kirst, et al., ed., Marcel Dekker, 2001, 624 pp., hard cover

BIOSYNTHESIS
d-(L-a-Aminoadipyl)-L-Cysteinyl-D-Valine Synthetase as a Model Tripeptide Synthetase
Metabolic Engineering for Cephalosporin C Yield Improvement and Production of Intermediates
Bioconversion of Penicillis to Cephalosporins
Direct Fermentative Production of Acyltylosins by Genetically Engineered Strains of Streptomyces fradiae
Engineering Streptomyces avermitilis for the Production of Novel Avermectins: Mutant Design and Titer Improvement
BIOCATALYSIS
Biocatalytic Syntheses of Chiral Intermediates for Antihypertensive Drugs
Cloning, Structure, and Activity of Ketone Reductases from Baker’s Yeast
Cross-Linked Enzyme Crystals: Biocatalysts for the Organic Chemist
Enzymatic Deacylation of Echinocandins and Related Antifungal Agents
SCREENING/OPTIMIZATION
Roles of Enzymes in Antibacterial Drug Discovery
Penicillin-Binding Proteins as Antimicrobial Targets: Expression, Purification, and Assay Technologies
Development of a High-Throughput Screen for Streptococcus pneumoniae UDP-N-Acetylmuramoyl-Alanine: D-Glutamate Ligase (MurD) for the Identification of MurD Inhibitors
Purification and Assay Development for Human Rhinovirus Proteases
Screening for Parasiticides Using Recombinant Microorganisms
Screening for Inhibitors of Lipid Metabolism
Design and Development of a Selective Assay System for the Phospholipase A₂ Superfamily
EMERGING TECHNOLOGIES
Understanding and Exploiting Bacterial Polyketide Synthases
Polyketide Synthases: Analysis and Use in Synthesis
Enzymatic Synthesis of Fungal N-Methylated Cyclopeptides and Depsipeptides
NEW STRATEGIES
for Target Identification, Validation, and Use of Enzymes in High-Throughput Screening
Use of Genomics for Enzyme-Based Drug Discovery
Assigning Precise Function to Genes
Redesigning Binding and Catalytic Specificities of Enzymes
PROTEOMICS
Chromatographic Fractionation Prior to Two-Dimensional Polyacrylamide Gel Electrophoresis for Enrichment of Low-Abundance Proteins to Facilitate Identification by Mass Spectrometric Methods