E4011
Sigma

ExtrAvidin^®−R-Phycoerythrin

buffered aqueous solution

MDL number

MFCD00163600

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Descriptions

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

The ExtrAvidin-R-Phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.

General description

ExtrAvidin, a uniquely modified avidin reagent, combines the high specific activity and sensitivity of avidin with the low background staining of streptavidin. Avidin consists of four identical subunits, each containing a single biotin binding site.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

form

buffered aqueous solution

extent of labeling

1-4 mol PE per mol ExtrAvidin®

application(s)

flow cytometry: 10 μL using 10⁶ human peripheral blood lymphocytes

storage temp.

2-8°C