E7525
Sigma

Anti-Ephrin-B2 antibody produced in goat

affinity isolated antibody, lyophilized powder

MDL number

MFCD02096317

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Descriptions

Immunogen

recombinant mouse ephrin-B2, expressed in NSO cells.

Specificity

By immunoblotting and ELISA, the antibody shows ~50% cross-reactivity with recombinant human ephrin-B5 and 15% cross-reactivity with mouse ephrin-B1.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Properties

antibody form

affinity isolated antibody

form

lyophilized powder

species reactivity

mouse

application(s)

immunoblotting: 0.1-0.2 μg/mL

indirect ELISA: 0.5-1.0 μg/mL

Gene Information

mouse ... Efnb2(13642)

storage temp.

−20°C

References

Reference

Bennett, B.D., et al., Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk. Proc. Natl. Acad. Sci. USA 92, 1866-1870, (1995) abstract

Inada, T., et al., Selective expression of the receptor tyrosine kinase, HTK, on human erythroid progenitor cells. Blood 89, 2757-2765, (1997) abstract

Wang, H.U., et al., Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell 93, 741, (1998) abstract

Vogt, T., et al., Overexpression of Lerk-5/Eplg5 messenger RNA: a novel marker for increased tumorigenicity and metastatic potential in human malignant melanomas. Clin. Cancer Res. 4, 4, (1998)