Enzyme Assays: A Practical Approach, 2nd ed. 

This edition combines revised chapters with new contributions in describing the design and execution of enzyme assays. Topics include protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. Theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high-throughout screening; statistical analysis of enzyme kinetic data; and determination of active site concentration