E9152
Sigma

Eotaxin-2 from mouse

>95% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder

Synonym

CCL24

MPIF-2

MDL number

MFCD01866306

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Descriptions

Analysis Note

The biological activity is measured by its ability to induce chemotaxis of rat Y3 cells transfected with the CCR3 receptor.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA containing 1.25 mg bovine serum albumin.

Biochem/physiol Actions

Eotaxin-2 is a potent eosinophil chemotactic factor, which is involved in homeostatic, allergen-induced, and IL-4 related immune responses. Eotaxin-2 exhibits 40%, 42%, and 39% amino acid identity to MCP-3, MIP-1α, and eotaxin, respectively. Mature mouse and human eotaxin-2 share approximately 57% amino acid sequence identity.

Properties

recombinant

expressed in Escherichia coli

assay

>95% (SDS-PAGE)

form

lyophilized powder

mol wt

predicted mol wt ~10.6 kDa

total impurities

Endotoxin, tested

Gene Information

mouse ... Ccl24(56221)

storage temp.

−20°C

References

Reference

Zimmermann, N.,, Murine eotaxin-2: a constitutive eosinophil chemokine induced by allergen challenge and IL-4 overexpression. J. Immunol. 165, 5839, (2000)

Safety

WGK Germany