EphB1/Fc Chimera from rat 

The biological activity is measured by its ability to bind recombinant mouse ephrin-B1/Fc in an ELISA assay.

Member of Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A3, ephrin-A4, ephrin-B1, ephrin-B2, and ephrin-B3; involved in pattern formation and morphogenesis. EphB1 can be detected in endothelial cells and is activated by ephrin-B1, initiating the assembly of endothelial cells into capillary-like cords.

Extracellular domain of rat EphB1 (amino acids 1-549) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker.

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.5.

expressed in mouse NSO cells

>95% (SDS-PAGE)

lyophilized powder

monomer calculated mol wt 85.6 kDa

mol wt ~110 kDa by SDS-PAGE (reducing)

Endotoxin, tested

rat ... Ephb1(24338)

−20°C

Flanagan, J.G., Vanderhaegen, P., The ephrins and Eph receptors in neural development. Annu. Rev. Neurosci. 21, 309, (1998)

Pasquale, E.B., The Eph family of receptors. Curr. Opin. Cell Biol. 9, 608, (1997)

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