E9653
Sigma

Monoclonal Anti-Endothelial Cells antibody produced in mouse

clone P1H12, purified immunoglobulin, buffered aqueous solution

Synonym

Anti-CD146

MDL number

MFCD03792419

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Descriptions

Immunogen

human umbilical cord cells (HUVEC).

Specificity

Recognizes cultured microvascular and large-vessel endothelial cells (MVEC and HUVEC, respectively) and endothelial cells in tissues and in circulation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Properties

antibody form

purified immunoglobulin

clone

P1H12

form

buffered aqueous solution

mol wt

antigen mol wt 130 kDa

species reactivity

human

application(s)

flow cytometry: suitable using 2% paraformaldehyde 9:1 methanol

immunocytochemistry: suitable using 4% paraformaldehyde 0.1-0.5% triton X-100, smears or live cells

immunohistochemistry: suitable using using acetone-fixed sections of human tongue

immunoprecipitation: suitable

indirect ELISA: suitable

microarray: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

References

Reference

Solovey, A., et al. New Engl. J. Med. 337, 1584, (1997) abstract

Lin, Y., et al. J. Clin. Invest. 105, 71, (2000) abstract

St Croix, B., et al. Science 289, 1197, (2000) abstract

Safety

Safety Statements

23-24/25

WGK Germany

NWG