Ephrin-A1/Fc Chimera from mouse 

The biological activity is measured by its ability to bind recombinant mouse EphA2/Fc in ELISA.

Member of ephrin ligand family that binds Eph receptor tyrosine kinases (EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7 and EphB1). Involved in pattern formation and morphogenesis. Ephrin-A1 can be detected on fetal osteoblasts, odotoblasts, chondrocytes, and squamous epithelium. Ephrin-A1 is expressed during embryonic vasculogenesis and during tumor-induced angiogenesis.

Extracellular domain of mouse ephrin-A1 (amino acids 1-182) fused to the C-terminal 6X histidine-tagged Fc region of human IgG.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

expressed in mouse NSO cells

>90% (SDS-PAGE)

lyophilized powder

monomer calculated mol wt 46.8 kDa

mol wt 50-55 kDa by SDS-PAGE (reducing)

endotoxin, tested

mouse ... Efna1(13636)

−20°C

Flanagan, J.G., Vanderhaegen, P., The ephrins and Eph receptors in neural development. Annu. Rev. Neurosci. 21, 309, (1998)

Takahashi, H., Ikeda, T., Molecular cloning and expression of rat and mouse B61 gene: implications of organogenesis. Oncogene 11, 879, (1995)

Ogawa, K., et al., The ephrin-A1 ligand and its receptor, EphA2, are expressed during tumor neovascularization. Oncogene 19, 6043-6052, (2000)

McBride, J.L., and Ruiz, J.C., Ephrin-A1 is expressed at sites of vascular development in the mouse. Mech. Dev. 77, 201-204, (1998)

Pasquale, E.B., The Eph family of receptors. Curr. Opin. Cell Biol. 9, 608, (1997)