Components
3 mL ExtrAvidin®-Alkaline Phosphatase
3 mL Biotinylated Secondary Antibodies
Complete Instructions and Assay Protocols
Features and Benefits
Use in immunohistology, ELISA, and immunoblotting assays.
Monoclonal Anti-Goat IgG antibodies in EXTRA-1 show no cross-reactivity with human IgG. These antibodies also recognize sheep IgG.
Affinity Isolated Antibodies in EXTRA-2A and EXTRA-3A and EXTRA-2, EXTRA-3 and EXTRA-6 have been adsorbed with human IgG and IgM to minimize cross-reactivity.
Biotinylated antibodies contain a spacer which improves accessibility for the ExtrAvidin® conjugates.
General description
These kits comprise universal reagents for use with primary antibodies in immunohistology, ELISA, and immunoblotting.
ExtrAvidin® is a unique form of avidin, available only from Sigma, that combines the high specificity and affinity of avidin for biotin with low non-specific binding at physiological pH. ExtrAvidin® alkaline phosphatase exhibits high sensitivity with low background.
Legal Information
ExtrAvidin is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.