Monoclonal Anti-CD62E (E-Selectin)−FITC antibody produced in mouse 

CD62E (E-Selectin) surface antigen is a glycoprotein and member of the selectin family of adhesion receptors. It is expressed by activated human endothelial cells and is detectable in the plasma.

TNF-α activated human endothelial cells.

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Recognizes the human CD62E (E-Selectin) surface antigen. The antibody may be used in flow cytometry, ELISA, immunoblotting, immunoprecipitation, and immunohistochemistry. It inhibits adhesion of neutrophils, eosinophils, and a skin-homing T-cell subpopulation to activated endothelial cells and CD62E-transfected cells.
5th Workshop: code no. E 040

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

purified immunoglobulin

1.2B6

buffered aqueous solution

antigen mol wt 100-115 kDa

human, pig

flow cytometry: 10 μL using 2 × 10⁵ cells

IgG1

human ... SELE(6401)

wet ice

2-8°C

Wellicome, S.M. J. Immunol. 144, 2558, (1990)

Picker, L.Y., et al. Nature 349, 796, (1991)

Schlossman, S.F. Leucocyte Typing V Oxford , (1995)

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