F1777
Sigma

Monoclonal Anti-HLA-DQ−FITC antibody produced in mouse

clone HK19, purified immunoglobulin, buffered aqueous solution

Synonym

Monoclonal Anti-HLA-DQ

Descriptions

Antigen Background

HLA-DQ molecules are bimolecular glycoprotein complexes of 29 kDa and 33 kDa components. The HLA-DQ antigens are present on peripheral blood lymphocytes and tonsillar and splenic mononuclear cells.

Immunogen

GM-1500 human tumor cell line.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Specificity

Recognizes MHC Class II molecules that are preferentially expressed on the surface of B lymphocytes. The antibody reacts weakly with resting or ConA-activated T cells, monocytes, granulocytes, and erythrocytes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Properties

antibody form

purified immunoglobulin

clone

HK19

form

buffered aqueous solution

species reactivity

human

application(s)