Fructose-6-phosphate Kinase, Pyrophosphate-dependent from potato tuber 

These two pyrophosphate-dependent fructose-6-phosphate kinases differ in their response to the physiological activator fructose 2,6-diphosphate [Fru (2,6)-P₂]. The enzyme from Propionibacterium freudenreichii (F8381) reportedly is not affected by Fru (2,6)-P₂.¹ The enzyme from potato tubers (F2258) is stimulated by Fru (2,6)-P₂ (half-maximal activation at 5.5 nM Fru (2,6)-P₂ for potato tuber enzyme).^1,2

One unit will convert 1.0 μmole of pyrophosphate and fructose 6-phosphate to fructose 1,6-diphosphate and inorganic phosphate per min at pH 8.0 at 30 °C in the presence of 1μM fructose 2,6-diphosphate and 17 mM glucose 6-phosphate.

Lyophilized powder containing sodium phosphate buffer and stabilizers with traces of inorganic pyrophosphate and dithiothreitol

lyophilized powder

Protein, ~30% Lowry

−20°C

1. VanSchaftingen, E., et al. Eur. J. Biochem. 129, 191, (1982)

2. Sabularse, D.C. and Anderson, R.L. Biochem. Biophys. Res. Commun. 103, 848, (1981)

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