F2922
Sigma

Monoclonal ANTI-FLAG^® BioM5 from mouse

clone M5, purified immunoglobulin, buffered aqueous solution

Synonym

Monoclonal ANTI-FLAG M5^® Biotin conjugate from mouse

MDL number

MFCD01633351

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Descriptions

Application

For immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.

Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca²⁺-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Features and Benefits

ANTI-FLAG M5 − Biotin conjugate has a high affinity for N-terminal Met-FLAG fusion proteins.

Other Notes

To view FLAG^® technology literature, visit www.sigma-aldrich.com/flagliterature.

Physical form

Solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 0.02% sodium azide

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody form

purified immunoglobulin