Monoclonal Anti-β-Actin–FITC antibody produced in mouse 

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

To view an Actin antibody selection guide, please click here.

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

purified immunoglobulin

AC-15

buffered aqueous solution

antigen mol wt 42 kDa

human, bovine, sheep, pig, rabbit, feline, canine, mouse, rat, guinea pig, chicken, carp, Hirudo medicinalis

Dictyostelium discoideum

direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts

IgG1

mouse ... Actb(11461)

human ... ACTB(60)

rat ... Actb(81822)

dry ice

−20°C

Gimona, M., et al., β-actin specific monoclonal antibody. Cell Motil. Cytoskeleton 27, 108-116, (1994)

North, A.J., et al., Calponin is localized in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J. Cell Sci. 107, 437-444, (1994)

Liao, J., et al., Direct reprobing with anti-β-actin antibody as an internal control for western blotting analysis. Biotechniques 28, 216, (2000)

North, A.J., et al., Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell Sci. 107, 445-455, (1994)

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