Monoclonal Anti-CD16−FITC antibody produced in mouse 

CD16 antigen (also known as the low affinity receptor for complexed IgG, or Fcγ-RIII) expressed on natural killer (NK) cells, a macrophage sub-population and weakly on granulocytes. The human CD16 molecule exhibits two truncated Ig-like domains. In NK cells and macrophages, a trans-membrane form (50-80 kDa) with a 25 amino acid cytoplasmic tail is present. This form is non-covalently associated with other receptors (FcεRIγ or the TcRξ chain). The human CD16 molecule in granulocytes is a glycosyl-phosphatidyl-inositol (GPI)-linked form.

viable human polymorphonuclear cells.

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Recognizes the human CD16 antigen expressed on natural killer (NK) cells, granulocytes and a macrophage subpopulation. The antibody reacts with both NA1 and NA2 neutrophils and NK lymphocytes. It strongly labels neutrophils in tissue frozen sections. Liver Kupffer cells are weakly stained. The epitope recognized by the antibody seems to reside near the IgG binding site of FCγ-RIII. The antibody functionally blocks binding of soluble immune complexes to granulocytes and inhibits E-IgG rosettes. It induces Ca²⁺ increase in neutrophils.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

purified immunoglobulin

3G8

buffered aqueous solution

human

protect from light

flow cytometry: 10 μL using 1 × 10⁶ cells

IgG1

human ... FCGR3A(2214), FCGR3B(2215)

wet ice

2-8°C

Fleit, H.B., et al., Knapp, W. Leucocyte Typing IV Oxford , (1989), 579

Schlossman, S.F., et al. Leucocyte Typing V Oxford , (1995), 805

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