F4042
Sigma

Monoclonal ANTI-FLAG^® M5 from mouse

clone M5, purified immunoglobulin, buffered aqueous solution

MDL number

Descriptions

Application

Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca²⁺-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Other Notes

To view FLAG^® technology literature, visit www.sigma-aldrich.com/flagliterature.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody form

purified immunoglobulin

clone

form

buffered aqueous solution

application(s)

immunoblotting (chemiluminescent): 10 μg/mL

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany

Monoclonal ANTI-FLAG® M5 from mouse

clone M5, purified immunoglobulin, buffered aqueous solution

Monoclonal ANTI-FLAG^® M5 from mouse