Monoclonal Anti-HLA Class I Antigen−FITC antibody produced in mouse 

human tonsil leukocyte membrane preparation.

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.
4th Workshop: code no. P1
5th Workshop: code nos. BP166, BP288, BP407, B 005

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

purified immunoglobulin

W6/32

buffered aqueous solution

human, monkey

direct immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections.

flow cytometry: 10 μL using 1 × 10⁶ cells

IgG2a

human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)

wet ice

2-8°C

Barnstable, C.J. Cell 14, 9, (1978)

Brodsky, F.M. Immunol. Rev. 47, 3, (1979)

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