Fluorescein isothiocyanate isomer I 

Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

≥90% (HPLC)

powder

λ_ex 492 nm; λ_em 518 nm (green)

>360 °C(lit.)

suitable for protein labeling

2-8°C

, E. Harlow and D. Lane, ed. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1988), 353-355

Der-Balian, G.P., FITC labelling of Fab'. Anal. Biochem. 173, 59, (1988)

Wilderspin, A.F., Green, N.M., Modification of thiol groups. Anal. Biochem. 132, 449, (1983)

Muramoto, K., Microsequencing of proteins and peptides (HPLC). Anal. Biochem. 141, 446, (1984)

Miller, L., Protein modification of actin at lysine-61. Eur. J. Biochem. 174, 23, (1988)

Aldrich MSDS 1, 948:C / Corp MSDS 1 (1), 1688:D / FT-IR 2 (3), 4153:C / FT-IR 1 (2), 469:C / FT-NMR 1 (2), 1560:B / IR-Spectra (3), 1142:D / IR-Spectra (2), 995:C / NMR-Reference 2 (2), 440:C / RegBook 1 (2), 2157:I / Sigma FT-IR 1 (2), 148:B / Stains and Dyes Ref, 377 / Structure Index 1, 445:C:5

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