Monoclonal Anti-CD62L (L-Selectin)−FITC antibody produced in mouse 

CD62L, a glycoprotein and member of the selectin family of adhesion receptors, is constitutively expressed on all classes of leukocytes including lymphocytes, monocytes, and polymorphonuclear cells. It is expressed on myeloid progenitor cells, erythroid precursor cells, and some thymocytes.

human PHA-activated peripheral blood mononuclear cells.

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Recognizes the CD62L (L-Selectin, LAM-1, Leu8, TQ1, LECAM-1, LECCAM-1, DREG) surface antigen. The antibody recognizes an epitope localized to the lectin domain. It inhibits “rolling” interactions and significantly reduces the binding of FITC-yeast phosphomannan monoester core polysaccharide to lymphocytes, monocytes and neutrophils.
5^th Workshop: Code No. S061

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

purified immunoglobulin

FMC46

buffered aqueous solution

antigen mol wt 74-95 kDa

human

flow cytometry: 10 μL using 1 × 10⁶ cells

IgG2b

human ... SELL(6402)

wet ice

2-8°C

Schlossman, S.F., et al. Leucocyte Typing V Oxford , (1995), 1499

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