F7425
Sigma

ANTI-FLAG^® from rabbit

affinity isolated antibody, buffered aqueous solution

MDL number

MFCD02262912

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Descriptions

Frequently Asked Questions

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Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.

Immunogen

Synthetic FLAG sequence containing peptide DYKDDDDK-GC conjugated through a terminal cysteine thiol to maleimide-activated KLH.

Other Notes

To view FLAG^® technology literature, visit www.sigma-aldrich.com/flagliterature.

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

application(s)

immunoblotting (chemiluminescent): 2.5 μg/mL using an Escherichia coli periplasmic extract expressing an N-terminal FLAGfusion protein

indirect immunofluorescence: 5 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany

Related Categories

... Molecular Biology > Antibodies to Peptide and Protein Fusion Tags

... Alphabetical Index > F

... Antibodies and Conjugates > FLAG

... FLAG System > FLAG Antibodies

F7425 - Datasheet (67kb)

Proteomics Prod Guide (140kb)

MSDS

Certificate of Analysis

Certificate of Origin

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F7425 (Sigma)

ANTI-FLAG® from rabbit

affinity isolated antibody, buffered aqueous solution

ANTI-FLAG^® from rabbit