Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)−FITC antibody produced in mouse 

synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop.

The antibody reacts specifically with the diphosphorylated form of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

purified immunoglobulin

MAPK-YT

buffered aqueous solution

antigen ERK-1 mol wt 44 kDa

antigen ERK-2 mol wt 42 kDa

human, rat, mouse, Drosophila, yeast, bovine, hamster, Xenopus, Caenorhabditis elegans

direct immunofluorescence: 1:20 using cultured human foreskin fibroblasts (paraformaldehyde-fixation)

IgG1

mouse ... Mapk1(26413), Mapk3(26417)

rat ... Mapk1(116590), Mapk3(50689)

human ... MAPK1(5594), MAPK3(5595)

dry ice

−20°C

Yao, Z., et al., Detection of partially phosphorylated forms of ERK by monoclonal antibodies reveals spatial regulation of ERK activity by phosphatases. FEBS Lett. 468, 37-42, (2000)

Yung, Y., et al., Detection of ERK activation by a novel monoclonal antibody. FEBS Lett. 408, 292-296, (1997)

3