Fructose-6-phosphate Kinase, Pyrophosphate-dependent from Propionibacterium freudenreichii (shermanii) 

These two pyrophosphate-dependent fructose-6-phosphate kinases differ in their response to the physiological activator fructose 2,6-diphosphate [Fru (2,6)-P₂]. The enzyme from Propionibacterium freudenreichii (F8381) reportedly is not affected by Fru (2,6)-P₂.¹ The enzyme from potato tubers (F2258) is stimulated by Fru (2,6)-P₂ (half-maximal activation at 5.5 nM Fru (2,6)-P₂ for potato tuber enzyme).²

One unit will convert 1.0 μmole of pyrophosphate and fructose 6-phosphate to fructose 1,6-diphosphate and inorganic phosphate per min at pH 7.4 at 30 °C.

Contains imidazole salts and stabilizer

Can be used for the determination of pyrophosphate by the method of O'Brien, W., Anal. Biochem., FB76, 423 (1976).

lyophilized powder

Protein, ~15% biuret

−20°C

1. VanSchaftingen, E., et al. Eur. J. Biochem. 129, 191, (1982)

2. Sabularse, D.C. and Anderson, R.L. Biochem. Biophys. Res. Commun. 103, 848, (1981)

3