F8775
Sigma

Formaldehyde solution

for molecular biology, 36.5-38% in H₂O

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Synonym

Formalin

Molecular Formula

HCHO

Molecular Weight

30.03

CAS Number

50-00-0

Beilstein Registry Number

1209228

MDL number

MFCD00003274

PubChem Substance ID

24894976

EG/EC Number

200-001-8

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Descriptions

Analysis Note

Tested for use in formaldehyde-agarose gel electrophoresis of RNA.

Application

Formaldehyde is used as a denaturant in formaldehyde-agarose gel electrophoresis of RNA. A gel concentration of 2.2 M formaldehyde is typically used. The formaldehyde concentration as supplied is approximately 12.3 M.

Properties

grade

for molecular biology

vapor density

1.03 (vs air)

vapor pressure

52 mmHg ( 37 °C)

autoignition temp.

572 °F

contains

10-15% methanol

concentration

36.5-38% in H₂O

density

1.09 g/mL at 25 °C(lit.)

References

Reference

Lehrach, H., et al., RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16, 4743-4751, (1977) abstract

, Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Plainview, NY , (1989), 7.43-7.45

Miller, K., Gel electrophoresis of RNA. Bethesda Res. Lab. Focus 9, 14, (1987)

Merck

Merck 13,4259

Beilstein

Beil. 1,IV,3017

Fieser

Fieser 1,397 / Fieser 2,200 / Fieser 4,238 / Fieser 5,312 / Fieser 6,264 / Fieser 7,158 / Fieser 8,231 / Fieser 9,224 / Fieser 10,186 / Fieser 11,240 / Fieser 12,232 / Fieser 13,136 / Fieser 14,168 / Fieser 15,161 / Fieser 16,171

reference

Corp MSDS 1 (1), 1733:C / FT-IR 2 (1), 730:A / FT-IR 1 (1), 465:A / IR-Spectra (3), 276:A / IR-Spectra (2), 248:A / RegBook 1 (1), 509:A / Sax 6, 1451 / Structure Index 1, 73:A:1 / Vapor Phase 3, 572:B

Safety

Hazard Codes

Risk Statements

23/24/25-34-40-43

Safety Statements

26-36/37/39-45

RIDADR

UN 1198 3/PG 3

WGK Germany

RTECS

LP8925000

UEL