F8924
Sigma

Fibroblast Growth Factor 10 human

>97% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder, suitable for cell culture

Synonym

FGF-10

MDL number

MFCD01867039

Expand/Collapse All

Descriptions

Analysis Note

The biological activity is measured by its ability to stimulate the proliferation of a monkey epithelial cell line 4MBr-5.

Biochem/physiol Actions

Holds unique roles in the brain, lung development, wound healing, and limb bud formation; mitogenic for epithelial and epidermal cells but not fibroblasts.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20�mM MOPS, 50�mM sodium sulfate, 1�mM DTT, 0.5�mM EDTA, pH�7.0, containing 50�μg bovine serum albumin per 1�μg cytokine.

Properties

recombinant

expressed in Escherichia coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

mol wt

mol wt 19 kDa by SDS-PAGE

total impurities

Endotoxin, tested

suitability

suitable for cell culture

Gene Information

human ... FGF10(2255)

storage temp.

−20°C

References

Reference

Rubin, J.S., et al., Purification and characterization of a newly identified growth factor specific for epithelial cells. Proc. Natl. Acad. Sci. USA 86, 802-806, (1989) abstract

Yamasaki, M., et al., Structure and expression of the rat mRNA encoding a novel member of the fibroblast growth factor family. J. Biol. Chem. 271, 15918-15921, (1996) abstract

Bellusci, S., et al., Fibroblast Growth Factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung. Development 124, 4867, (1997) abstract

Tagashire, S., Cloning of mouse FGF10 and up-regulation of its gene expression during wound healing. Gene 197, 197, (1997)

Ohuchi, H., et al., The mesynchymal factor, FGF10, initiates and maintains the outgrowth of the chick limp bud through interaction with FGF8, an apical ectodermal factor. Development 124, 2235-2244, (1997) abstract

Emoto, H., et al., Structure and expression of human fibroblast growth factor-10. J. Biol. Chem. 272, 23191-23194, (1997) abstract

Safety

WGK Germany