Fluorescamine 

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.^1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.³ Effectively blocks newly generated amino termini in protein sequence analyses.

>97% (TLC)

powder

off-white to yellow

153-157 °C(lit.)

acetone: 50 mg/mL

1. Lai, C.Y. Meth.Enzymol. 47, 236, (1977)

2. Udenfriend, S. Science 178, 871, (1972)

3. Sogowa, K., and Takahaski, K., Use of fluorescamine-labeled casein as a substrate for assay of proteinases. J. Biochem. Tokyo 83, 1783, (1978)

Stein, S., et al., Amino acid analysis with fluorescamine at the picomole level. Arch. Biochem. Biophys. 155, 202, (1973)

Bhown, A.S., et al. Anal. Biochem. 112, 158, (1981)

Merck 13,4184

FT-IR 2 (3), 4139:B / FT-IR 1 (2), 1009:D / FT-NMR 1 (3), 554:C / IR-Spectra (3), 1472:H / RegBook 1 (2), 2785:J / Sigma FT-IR 1 (2), 146:C / Stains and Dyes Ref, 371 / Structure Index 1, 443:C:1

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