Fundamentals of Light Microscopy and Electronic Imaging 

Researchers need new skills in order to use a modern research microscope, such as how to align microscope optics and perform image processing. This book explores the basics of microscope design and use. It discusses optical principles involved in diffraction and image formation in the light microscope, basic modes of light microscopy, components of modern electronic imaging systems, and image processing operations necessary to acquire and prepare an image. It also reviews such topics as Illuminators, filters, and isolation of specific wavelengths; Phase contrast and differential interference contrast; Fluorescence and confocal laser scanning microscopy; Digital CCD microscopy and image processing.