Fructose Assay Kit 

This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.

sufficient for 20 assays

2-8°C

Beuter, H.O. Meth. Enzym. Anal. 3rd ed., 6, 321, (1985)

Official Methods of Analysis of the AOAC 16th ed., Arlington, VA , (1995), 28.1.17

fructose standard 500 mg

glucose assay reagent 1 vial

phosphoglucose isomerase 1 vial