Free Glycerol Determination Kit 

The triglyceride and free glycerol kits are for the quantitative determination of glycerol, total triglycerides or free triglycerides.

In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.

The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5'-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5'-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H₂O₂). Peroxidase catalyzes the coupling of H₂O₂ with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.

1 kit sufficient for 1000 reactions

2-8°C

Xn

21/22-32-36

7-26-36/37