FLAG^® Immunoprecipitation Kit 

Sigma’s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with ANTI-FLAG^®-M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

• Utilizes highly specific ANTI-FLAG M2 affinity gel
• No preliminary steps or calibrations
• 3X FLAG^™ peptide provides efficient and gentle elution through direct competition

ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

3xFLAG is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

1 kit sufficient for 50 reactions

wet ice

−20°C

C2978, CelLytic^™ M

F3165, Monoclonal ANTI-FLAG^® M2 antibody produced in mouse

ANTI-FLAG M2 affinity gel 1 mL A2220

FLAG-BAP™ control protein 50 μg P7582

3X FLAG peptide 1 mg F4799