Mammalian Amino-terminal FLAG^® Transient Expression Kit 

The Mammalian Amino-Terminal FLAG^® Transient Expression Kit provides all of the specialized FLAG components needed to perform expression of a recombinant N-terminal FLAG fusion in mammalian cells. The pFLAG-CMV^™-1 (E7273) and pFLAG-CMV-2 (E7398) Expression Vectors allow transient expression of secreted or cytoplasmically expressed fusion proteins. Also provided in the kit are controls for expression, detection, and affinity purification.

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

pFLAG-CMV1 is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

wet ice

−20°C

ANTI-FLAG M2 affinity gel 1 mL

ANTI-FLAG M2 monoclonal antibody 200 μg F3165

ANTI-FLAG M5 monoclonal antibody 200 μg F4042

C-CMV-24 sequencing primer 1 μg P5475

N-CMV-30 sequencing primer 1 μg

FLAG peptide 4 mg F3290

Met-FLAG-BAP control protein 100 μg P5975

pFLAG-CMV-2 expression vector (no leader) 10 μg E7398

pFLAG-CMV-1 expression vector (preprotrypsin leader) 10 μg