FluoroProfile^® Protein Quantification Kit 

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Reversible covalent binding to proteins by the fluorophore epicocconone provides a powerful and sensitive method for protein quantification

• Yields an intense fluorescent red on protein binding
• Binding is completely reversible making the protein suitable for mass spectrometry and functional assays
• Water soluble proteins may be quantified without using organic solvents
• A large dynamic range, large Stokes shift and highly sensitive
• Highly tolerant of substances commonly interfering with protein quantification
• Biodegradable and environmentally friendly

For the quantification of total protein in solution by the interaction of a unique fluorophore with the amines of polypeptides.

FluoroProfile is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

−20°C

Philip J. L. Bell and Peter Karuso, Epicocconone, A Novel Fluorescent compound from the Fungus Epicoccum nigrum J. Am. Chem. Soc. 125, 9304, (2003) abstract

UN 3316 9/PG 2

FluoroProfile^® Fluorescent Reagent

Quantitation Buffer

Protein Standard, 2 mg/vial P5619