G0296
Sigma

Anti-GRK2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym

Anti-β-Adrenergic receptor kinase-1

Anti-β-ARK1

Anti-ADRBK1

Anti-BARK1

Anti-G Protein-dependent receptor kinase 2

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Descriptions

Immunogen

Synthetic peptide corresponding to amino acids 674-689 located at the C-terminus of human GRK2/βARK1, conjugated to KLH. The immunizing sequence is specific to GRK2 (not found in human GRK1, and GRK3-7). It is identical in dog GRK2 and highly conserved (single amino acid substitution) in rat, mouse, and bovine GRK2/βARK1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

mol wt

antigen mol wt ~80 kDa

species reactivity

human

application(s)

immunoblotting: 0.5-1 μg/mL using HEK-293T cells expressing human GRK2 or Jurkat cell lysate

Gene Information

human ... ADRBK1(156)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Pitcher, J.A., et al., G protein-coupled receptor kinases. Annu. Rev. Biochem. 67, 653-692, (1998) abstract

Penela, P., et al., Mechanisms of regulation of the expression and function of G protein-coupled receptor kinases. Cell. Signal. 15, 973-981, (2003) abstract

Ren, X-R., et al., Different G protein-coupled receptor kinases govern G protein and beta-arrestin-mediated signaling of V2 vasopressin receptor. Proc. Natl. Acad. Sci. USA 102, 1448-1453, (2005) abstract

Aragay, A.M., et al., G protein-coupled receptor kinase 2 (GRK2): mechanisms of regulation and physiological functions. FEBS Lett. 430, 37-40, (1998) abstract

Pitcher, J.A., et al., Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. J. Biol. Chem. 274, 34531-34534, (1999) abstract

Safety

WGK Germany