Granulocyte Colony-Stimulating Factor 

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

Granulocyte-colony stimulating factor is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with INFγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G₀ period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Lyophilized from 10 mM acetic acid containing 250 μg bovine serum albumin

from human

expressed in Escherichia coli

≥95% (SDS-PAGE and N-terminal analysis)

mol wt 18.8 kDa (175 amino acids including N-terminal methionine)

Endotoxin, tested

cell culture tested

−20°C

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Heim, M., The Jak-STAT pathway: cytokine signaling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19, 75, (1999)

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Risau, W., et al. EMBO J. 5, 3179, (1986)

Seulberger, H., et al. EMBO J. 9, 2151, (1990)

Quesniaux, V.F.J., Jones, T.C. The Cytokine Handbook 3rd ed., San Diego, CA , (1998), 637

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Smith, L.R., et al. Immunogenetics 39, 80, (1994)

Nicola, N.A., Nicola, N.A., ed. Guidebook to Cytokines and Their Receptors NY, NY , (1994), 171

3

G1029, Monoclonal Anti-Granulocyte Colony Stimulating Factor antibody produced in mouse

G5171, Anti-Granulocyte Colony Stimulating Factor antibody produced in goat

G0540, Monoclonal Anti-Granulotyte Colony Stimulating Factor antibody produced in rat

G5421, Monoclonal Anti-Granulocyte Colony Stimulating Factor antibody produced in rat

G5296, Anti-Granulocyte Colony Stimulating Factor antibody produced in goat

G5046, Anti-Granulocyte Colony Stimulating Factor antibody produced in goat

G4921, Anti-Granulocyte Colony Stimulating Factor antibody produced in goat