GenElute^™ Mammalian Genomic DNA Miniprep Kit 

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

For additional information, please see www.sigma-aldrich.com/genomicdna.

• Typical DNA yields of 25 μg from 2 × 10⁶ cultured cells or 30 μg from 25 mg of tissue (Fig. 4)
• Preparation time is only 20 minutes after lysis
• Purified genomic DNA has A₂₆₀/A₂₈₀ ratios between 1.6 and 1.9
• No need for mechanical homogenization
• 40% more purification preps offered than market leader

Mammalian cells and tissues are lysed with a chaotropic salt-containing buffer to ensure denaturation of macromolecules. DNA is bound to the spin column membrane and the remaining lysate is removed by centrifugation. A filtration column is used to remove cell debris, after washing to remove contaminants; the DNA is eluted with buffer into a collection tube. The purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction digestion (Fig. 3), ligation, and PCR.

GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

sufficient for 10 purifications

UN 3316 9/PG 3