G Proteins: Techniques of Analysis 

0-8493-3381-4

978-0-8493-3381-1

D.R. Manning, ed., CRC Press, 1999, 320 pp., hard cover

Preface Expression and Purification of G Protein a Subunits in E. scherichia coli -
Purification of Recombinant G Protein a and bg Subunits from Sf9 Cells-
Expression and Functional Analysis of G Protein a Subunits in Mammalian Cells...
Identification of Regulators and Targets of G Protein a Subunits Using the Yeast Two-Hybrid System-
Functional Analysis of G Protein bg Subunits...-
Immunological Techniques of Analysis-C. A. Chen and D. R. Manning GTPase-Activating Proteins (GAPs) for Heterotrimeric G Proteins-
Fatty Acid Acylation of G Protein a Subunits-
ADP-Ribosylation of G Proteins with Cholera Toxin or Pertussis Toxin-
Analysis of Receptor.G Protein coupling with PTX-Risistant Analogues of G(i in subscript) and G(o in subscript)-
Microinjection of Antibodies Neutralizing G. Protein Function-
Agonist-Promoted (35S)GTPgS-Binding as a Probe of Receptor-G Protein Communication in Reconstituted Sf9 Cells-
Methods of Sequestering bg in the Intact Cell-
In Vitro and In Vivo Analysis of Heterotrimeric G-Protein Signaling via DNA/RNA Antisense Strategies-