Gel Loading Buffer 

Bromophenol blue (0.05% w/v), sucrose (40% w/v), EDTA (0.1 M, pH 8.0), and SDS (0.5% w/v).

Gel loading buffer is especially formulated for non-denaturing polyacrylamide and agarose gel electrophoresis of nucleic acids. The gel loading solution contains a bromophenol blue tracking dye and sucrose to add density and facilitate sample loading. EDTA has been included to inhibit nucleases that require divalent cations and SDS has been added to help dissociate DNA-protein complexes which can interfere with electrophoresis.

With EDTA to inactivate nucleases

Recommended usage: add 1 volume gel loading solution to 2-5 volumes of sample and mix well.

RNAse, none detected

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