| G3041 Sigma |
Genetic Manipulation of Receptor Expression and Function |
General description
This review discusses principles and applications for genetic manipulation of receptor expression and function. It provides a roadmap to transgenic and knockout methodology, includes an explanation of the differences between embryonic stem cells and fertilized zygotes, clarifies use of a DNA construct to make a transgenic mouse and a targeting vector for a knockout. It covers changing aspects in transgenic mouse technology such as generation of transgenic mice using large DNA fragments, role of modifier genes, importance of behavioral testing in analyzing disease phenotypes, and growing role of antisense techniques in studies of gene function.
Table of Contents
Transgenic Rescue of Mutant Phenotypes Using Large DNA Fragments (S. Camper & T. Saunders).
Behavioral Characterization of Transgenic and Knockout Mice (J. Crawley).
Genetic Analysis of Receptor Phenotypes (S. Bultman, et al.).
Immortalization of Cell Lines from Transgenic and Knockout Mice by Viral Transformation (J. Chou).
The VP16-Dependent Binary System for Inducible Gene Expression in Transgenic Mice (C. Kappen).
Gene-Targeting Strategies in the Analysis of Alcohol and Anesthetic Mechanisms (G. Homanics).
In Vivo Functions of the c-Met Gene: (HGF/SF Receptor) (S. Giordano & P. Comoglio).
Tissue-Specific Knockouts of Insulin Receptor Tyrosine Kinase (R. Kulkarni, et al.).
Targeted Dominant Negative Mutations (W. LaRochelle, et al.).
Toxin-Mediated Targeted Cell Ablation In Vivo (J. Drago & J. Wong).
Antisense RNA-Mediated Inhibition of Gene Expression (H. Wang, et al.).
Delivery of Antisense Oligonucleotides to the Brain to Analyze Receptor Function (S. Kaur & I. Creese).
Index.
Behavioral Characterization of Transgenic and Knockout Mice (J. Crawley).
Genetic Analysis of Receptor Phenotypes (S. Bultman, et al.).
Immortalization of Cell Lines from Transgenic and Knockout Mice by Viral Transformation (J. Chou).
The VP16-Dependent Binary System for Inducible Gene Expression in Transgenic Mice (C. Kappen).
Gene-Targeting Strategies in the Analysis of Alcohol and Anesthetic Mechanisms (G. Homanics).
In Vivo Functions of the c-Met Gene: (HGF/SF Receptor) (S. Giordano & P. Comoglio).
Tissue-Specific Knockouts of Insulin Receptor Tyrosine Kinase (R. Kulkarni, et al.).
Targeted Dominant Negative Mutations (W. LaRochelle, et al.).
Toxin-Mediated Targeted Cell Ablation In Vivo (J. Drago & J. Wong).
Antisense RNA-Mediated Inhibition of Gene Expression (H. Wang, et al.).
Delivery of Antisense Oligonucleotides to the Brain to Analyze Receptor Function (S. Kaur & I. Creese).
Index.
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