G4142
Sigma

β-Galactosidase from bovine testes

ammonium sulfate suspension, 1-3 units/mg protein (modified Warburg-Christian)

Synonym

β-D-Galactoside galactohydrolase

CAS Number

9031-11-2

Enzyme Commission (EC) Number

3.2.1.23 ( BRENDA | IUBMB )

MDL number

MFCD00130623

EG/EC Number

232-864-1

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Descriptions

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH approx. 5.0

Properties

form

ammonium sulfate suspension

foreign activity

α- and β-glucosidase ≤0.2%

α-fucosidase ≤0.2%

α-galactosidase ≤0.2%

α-mannosidase ≤0.2%

β-N-acetylglucosaminidase ≤1.0%

storage temp.

2-8°C

References

Reference

Distler, J.J., The purification and properties of β-galactosidase from bovine testes. J. Biol. Chem. 248, 6772, (1973) abstract

Safety

WGK Germany

3

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Substrate

B4252, 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

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B4387, 5-Bromo-3-indolyl β-D-galactopyranoside

F2756, Fluorescein di(β-D-galactopyranoside)

M1633, 4-Methylumbelliferyl β-D-galactopyranoside

N9643, Naphthofluorescein di-(β-D-galactopyranoside)

Inhibitor

D6518, Diethylenetriaminepentaacetic acid

E4378, Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

ED2SS, Ethylenediaminetetraacetic acid disodium salt dihydrate

P9375, 1,10-Phenanthroline monohydrate

Related Categories

... Carbohydrate hydrolysis & PTM analysis > Carbohydrate and PTM Analysis

... Carbohydrate hydrolysis & PTM analysis > Carbohydrate Hydrolysis

... Carbohydrate-active Enzymes > Exoglycosidases

... 3.x.x.x Hydrolases > 3.2.x.x Glycosidases

Metabolic Pathways Chart (784 kb)

MSDS

Certificate of Analysis

Certificate of Origin

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G4142 (Sigma)

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