Galactosyltransferase from bovine milk 

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Lyophilized powder containing Tris, EDTA, and (NH₄)₂SO₄

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

lyophilized powder

Protein, ~50% modified Warburg-Christian

<10 % protein α-lactalbumin

−20°C

Schanbacher, F. and Ebner, K.E. J. Biol. Chem. 245, 5057, (1970) abstract

Fitzgerald, D.K., et al. J. Biol. Chem. 245, 2103, (1970) abstract

Narimatsu, H., et al. Proc. Natl. Acad. Sci. USA 83, 4720, (1986) abstract

Brew, K., et al. Proc. Natl. Acad. Sci. USA 59, 491, (1968) abstract

3