G5635
Sigma

β-Galactosidase from Escherichia coli

Grade VIII, lyophilized powder, 600-1,200 units/mg protein

Synonym

β-D-Galactoside galactohydrolase

Lactase

CAS Number

9031-11-2

Enzyme Commission (EC) Number

3.2.1.23 ( BRENDA | IUBMB )

MDL number

MFCD00130623

EG/EC Number

232-864-1

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Descriptions

Application

May be used as a positive control protein with anti-β-galactosidase antibodies.

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Physical form

Contains Tris buffer salts and magnesium chloride

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Enhanced Quality Profile

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Properties

type

Grade VIII

form

lyophilized powder

does not contain

BSA as standardization of activity

composition

Protein, ≥70% biuret

quality classification

Premium

storage temp.

−20°C

References

Reference

Fowler, A.V., et al., Amino acid sequence of beta-galactosidase XI; Peptide ordering procedures and the complete sequence. J. Biol. Chem. 253, 5521, (1978)

Safety

WGK Germany

RTECS

LW5840000