G6511
Sigma

Glutathione S-Transferase from equine liver

lyophilized powder, ≥25 units/mg protein

Synonym

Glutathione R-transferase

Glutathione S-alkenetransferase

Glutathione S-alkyltransferase

Glutathione S-aralkyltransferase

Glutathione S-aryltransferase

Glutathione S-epoxidetransferase

GST

CAS Number

50812-37-8

Enzyme Commission (EC) Number

2.5.1.18 ( BRENDA | IUBMB )

MDL number

MFCD00131198

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Descriptions

Analysis Note

Protein determined by biuret.
Purified and assayed by a modification of the method of Simons and Vander Jagt.
Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.

Unit Definition

One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.

Physical form

Lyophilized powder containing Tris and phosphate buffer salts, reduced glutathione and EDTA

Biochem/physiol Actions

Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.

Properties

form

lyophilized powder

mol wt

mol wt 45-50 kDa

composition

Protein, ~75%

storage temp.

−20°C

References

Reference

Asaoka, K., and Takahashi, K. J. Biochem. 82, 1313, (1977)

Simons, P.C. and Vander Jagt, D.L. Anal. Biochem. 82, 334, (1977)

Warholm, M., et al. Biochemistry 25, 4119, (1986)

Lee, C.-Y.G. Biochem. Soc. Trans. 12, 30, (1984)

Asoka, K. J. Biochem. 95, 685, (1984)

Habig, W.H. and Pabst, M.J. J. Biol. Chem. 249, 7130, (1974)

Safety

WGK Germany