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Descriptions
Analysis Note
The antibody may also be used in EMSA (electrophoretic mobility shift analysis).
Immunogen
rat growth factor independence-1 (GFI1).
Specificity
The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Properties
antibody form
purified immunoglobulin
clone
2.5D.17
form
buffered aqueous solution
mol wt
antigen mol wt ~55 kDa
species reactivity
human, rat, mouse
concentration
~2 mg/mL
application(s)
immunoblotting: 1-2 μg/mL using total cell extract of A549 cells
immunohistochemistry: suitable
microarray: suitable
isotype
IgG1
Gene Information
mouse ... Gfi1(14581)
rat ... Gfi1(24388)
human ... GFI1(2672)
shipped in
dry ice
storage temp.
−20°C
References
Reference
Kazanjian, A., et al. Cancer Res. 64, 68774-68782, (2004)
Doan, L.L., et al. Nucleic Acids Res. 32, 2508-2519, (2004) abstract
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