G7005
Sigma

Glutamic-Oxalacetic Transaminase from porcine heart

Type II-A, lyophilized powder, 100-400 units/mg protein

Synonym

L-Aspartate:2-oxoglutarate aminotransferase

Aspartate Aminotransferase

GOT

CAS Number

9000-97-9

Enzyme Commission (EC) Number

2.6.1.1 ( BRENDA | IUBMB )

MDL number

MFCD00131191

EG/EC Number

232-571-9

Expand/Collapse All

Descriptions

Analysis Note

Protein determined by biuret.

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.5 at 37 °C, in the presence of L-aspartic acid. One unit is equivalent to ~2,000O.D. (Karmen) units at 25 °C.

Physical form

Lyophilized powder containing approx. 20% sodium citrate buffer salt

Properties

type

Type II-A

form

lyophilized powder

composition

Protein, ~80%

foreign activity

glutamic-pyruvic transaminase <0.03%

lactic dehydrogenase and malic dehydrogenase <0.01%

storage temp.

−20°C

Safety

Safety Statements

22-24/25

WGK Germany

Related Categories

... Application Index > Diagnostic and Analytical Enzymes

... General Metabolic Enzymes > General Metabolic Enzymes A-H

... Plasma, Blood, and Related Proteins and Reagents > Glutamic-Oxalacetic Transaminase

... 2.x.x.x Transferases > 2.6.x.x Nitrogenous groups

Metabolic Pathways Chart (784 kb)

G7005 - Enzyme Assay (15kb)

MSDS

Specification Sheet

Certificate of Analysis

Certificate of Origin

More Information