G7163
Sigma

α-Galactosidase, positionally specific from Escherichia coli

recombinant, expressed in Escherichia coli, buffered aqueous solution

CAS Number

9025-35-8

Enzyme Commission (EC) Number

3.2.1.22 ( BRENDA | IUBMB )

MDL number

MFCD00130486

EG/EC Number

232-792-0

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Descriptions

Biochem/physiol Actions

Cleaves α(1→3)- and α(1→6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. It is particularly efficient for removing α-linked galactose under conditions where the pH must be neutral or above, for example, with live cells.

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl α-D-galactopyranoside per min at pH 6.5 at 25 °C.

Physical form

Solution in 20 mM Tris, pH 7.5, 25 mM NaCl

Properties

recombinant

expressed in Escherichia coli

form

buffered aqueous solution

activity

≥30 units/mg protein

Gene Information

Escherichia coli CFT073 ... melA(1037886)

storage temp.

2-8°C

References

Reference

Schmid K, Schmitt R., et al. Eur. J. Biochem. 67, 95-104, (1976) abstract