G7896
Sigma

β-Glucuronidase from Escherichia coli

Type X-A, lyophilized powder, 20,000,000-60,000,000 units/g protein, pH 6.8 (30 min assay)

Synonym

β-D-Glucuronide glucuronosohydrolase

CAS Number

9001-45-0

Enzyme Commission (EC) Number

3.2.1.31 ( BRENDA | IUBMB )

MDL number

MFCD00130629

EG/EC Number

232-606-8

Expand/Collapse All

Descriptions

Application

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Physical form

Highly purified lyophilized powder containing buffer salts and stabilizer

Properties

type

Type X-A

form

lyophilized powder

composition

Protein, ~30% biuret

shipped in

dry ice

storage temp.

−20°C

References

Reference

, Voigt, K.D. and Schmidt, H. Meth. Enzym. Anal. New York , (1974) 4

Merck

Merck 13,4480

Safety

WGK Germany

RTECS

LZ8990000