G8898
Sigma

Glycine

for electrophoresis, ≥99%

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Synonym

Aminoacetic acid

Aminoethanoic acid

Glycocoll

Molecular Formula

NH₂CH₂COOH

Molecular Weight

75.07

CAS Number

56-40-6

Beilstein Registry Number

635782

MDL number

MFCD00008131

PubChem Substance ID

24895355

EG/EC Number

200-272-2

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Descriptions

Analysis Note

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

Application

Glycine is a component of Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis¹. Glycine is also a component of Towbin′s transfer buffer for Western blots².

Biochem/physiol Actions

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Properties

grade

for electrophoresis

assay

≥99%

total impurities

≤0.01% Insoluble matter

pKa (25 °C)

2.35

240 °C (dec.)(lit.)

anion traces

chloride (Cl^-): ≤70 ppm

cation traces

heavy metals (as Pb): ≤20 ppm

abs.

A1M/280 ≤0.15

Gene Information

rat ... Grin2a(24409)

References

Cited Reference

1. Hames, B.D. and Rickwood, D. Gel Electrophoresis of Proteins: A Practical Approach 2nd ed., New York, NY , (1990), 32-35

2. Towbin, H., et al., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350, (1979) abstract

Reference

Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970) abstract

Merck

Merck 13,4504

Beilstein

Beil. 4,IV,2349

Fieser

Fieser 1,412

reference

Corp MSDS 1 (1), 1786:D / FT-IR 2 (1), 898:A / FT-IR 1 (1), 563:A / FT-NMR 1 (1), 867:A / Fenaroli, 307 / IR-Spectra (3), 338:A / IR-Spectra (2), 302:A / NMR-Reference 2 (1), 481:A / RegBook 1 (1), 645:A / Sax 6, 1481 / Sigma FT-IR 1 (1), 176:B / Structure Index 1, 94:A:1

Safety

Safety Statements

22-24/25

WGK Germany

RTECS

MB7600000