Golgi Isolation Kit 

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT0100).

sufficient for 50 g (tissue)

wet ice

2-8°C

Allan, V.J., and Vale, R.D., Cell cylce control of microtubule-based membrane transport and tubule formation in vitro. J. Cell Biol. 113, 347-359, (1991) abstract

Sjoberg, E.R., and Varki, A., Kinetic and spatial interrelationships between ganglioside glycosyltransferase(s) and O-acetyltransferase(s) in human melanoma cells. J. Biol. Chem. 268, 10185-10196, (1993) abstract

Wong, S.H., et al., Syntaxin 7, a novel syntaxin member associated with the early endosomal compartment. J. Biol. Chem. 273, 375-380, (1998) abstract

Roth, J., and Berger. E.G., Immunocytochemical localization of galactosyltransferase in HeLa cells: codistribution with thiamine pyrophosphatase in trans-Golgi cisternae. J. Biol. Chem. 93, 223-229, (1982)

Surroca, A., and Wolff, D., Inositol 1,4,5-tris-phosphate but not ryanodine-receptor agonists induces calcium release from rat liver Golgi apparatus membrane vesicles. J. Membr. Biol. 177, 243-249, (2000) abstract

Prchla, E., et al., Virus-mediated release of endosomal content in vitro: different behavior of adenovirus and rhinovirus serotype 2. J. Cell Biol. 131, 111-123, (1995) abstract

Isolation Buffer 5X 120 mL

2.3 M Sucrose Solution 120 mL

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL P8340