GlycoProfile^™ FLAG^®–Phosphine conjugate 

This reagent incorporates a reactive phosphine conjugated with the FLAG tag sequence for interaction with metabolically incorporated azido-sugars. It can enable detection and/or enrichment using a broad range of FLAG reagents that are conjugated to dye molecules or solid supports from Sigma-Aldrich. The FLAG system allows for highly specific interactions, avoiding off-target detection and unwanted contamination. This system also avoids undesirable toxic effects that may result in inaccurate representation of the glycoproteome.

GlycoProfile is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

dry ice

−20°C

Jennifer A. Prescher, Danielle H. Dube, Carolyn R. Bertozzi, Chemical remodelling of cell surfaces in living animals Nature 430, 871, (2004)

Eliana Saxon, Carolyn R. Bertozzi, Cell Surface Engineering by a Modified Staudinger Reaction Science 287, 2007, (2000) abstract

Carolyn R. Bertozzi, Laura L. Kiessling, Chemical Glycobiology Science 291, 2357, (2001) abstract

A7355, N-Azidoacetylglucosamine, Acetylated

A7605, N-Azidoacetylmannosamine, Acetylated

A7480, N-Azidoacetylgalactosamine, Acetylated