Glutathione Reductase Assay Kit 

UV assay: One unit will cause the oxidation of 1.0 μmole of NADPH at 25 °C at pH 7.5.
Colorimetric assay: One unit will cause the reduction of 1.0 μmole of DTNB to TNB at 25 °C at pH 7.5.

Glutathione reductase (EC 1.6.4.2) (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A₃₄₀ caused by the oxidation of NADPH or the increase in A₄₁₂ caused by the reduction of dithiobis(2-nitrobenzoic acid).

This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A₃₄₀ caused by the oxidation of NADPH or the increase in A₄₁₂ caused by the reduction of dithiobis(2-nitrobenzoic acid).

sufficient for 100 tests

human ... GSR(2936)

2-8°C

Dolphin D., et al., Glutathione: Chemical, Biochemical and Metabolic Aspects. Glutathione: Chemical, Biochemical and Metabolic Aspects, (1989)

Smith, I.K., et al., Assay of glutathione reductase in crude tissue homogenates using 5,5'-dithiobis(2-nitrobenzoic acid). Anal. Biochem. 175, 408-413, (1988) abstract

Factor, V.M., et al., Disruption of redox homeostasis in the transforming growth factor-alpha/c-myc transgenic mouse model of accelerated hepatocarcinogenesis. J. Biol. Chem. 273, 15846-15853, (1998) abstract

5,5′-Dithiobis(2-nitrobenzoic acid) 50 mg D8130

Glutathione, oxidized, disodium salt 100 mg G4626

Glutathione Reductase Assay Buffer 125 mL

Glutathione Reductase Dilution Buffer 100 mL

Glutathione Reductase Positive Control 1 vial

β-Nicotinamide adenine dinucleotide 25 mg N6505