H0148
Sigma

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse

clone IAP-9, ascites fluid

MDL number

Descriptions

Antigen Background

Heat Shock Protein 25 (HSP 25) is constitutively expressed in smooth muscle. It is also expressed in fibroblasts following heat shock.

Immunogen

partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

Specificity

A major proteolytic fragment of 22 kDa may also be detected. The antibody suppresses the inhibition of actin elongation induced by the 25 kDa IAP. The antibody displays a punctate or fine fibrillary pattern of labeling throughout the cytoplasm in heat-shocked cells and co-localization with mitochondria. Reactivity has been observed with HSP 25 of various organs.

Properties

antibody form

ascites fluid

clone

IAP-9

mol wt

antigen mol wt 25 kDa

contains

15 mM sodium azide

species reactivity

human, rabbit, guinea pig, chicken, turkey, rat, mouse, frog

application(s)

immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle

indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts

microarray: suitable

isotype

IgG1

Gene Information

mouse ... Hspb1(15507)

rat ... Hspb1(24471)

human ... HSPB1(3315)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Miron, T., et al. Eur. J. Biochem. 178, 543, (1988) abstract

Miron, T. J. Biol. Chem. 114, 255, (1991)

Safety

WGK Germany